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Purified Mouse Anti-Cdk1
Product Details
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BD Transduction Laboratories™
Cdc2, p34
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human p34 [cdc2] aa. 283-297
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Tested During Development)
34 kDa
250 µg/ml
AB_397454
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610037 Rev. 1
Antibody Details
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1/Cdk1/Cdc2

Cdk1, also known as p34 [cdc2], is a ubiquitously expressed serine/threonine protein kinase. Cdk1/cdc2 has been identified as the catalytic subunit of the maturation-promoting factor (MPF), while cyclin B acts as the regulatory subunit. The binding of these two subunits is critical to the transition into M-phase of the mammalian cell cycle, and this factor's role is regulated by a series of phosphorylations and dephosphorylations. After binding to cyclin B, cdk1/cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex. Phosphorylation of cdk1/cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the prophase/metaphase transition and completes activation of the cdc2/cyclin B complex, initiating the cell's entry into mitosis.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610037 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610037 Rev.1
Citations & References
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Development References (5)

  1. Barboule N, Lafon C, Chadebech P, Vidal S, Valette A. Involvement of p21 in the PKC-induced regulation of the G2/M cell cycle transition. FEBS Lett. 1999; 444(1):32-37. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Garcia JF, Camacho FI, Morente M, et al. Hodgkin and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue microarrays. Blood. 2003; 101(2):681-689. (Biology: Immunohistochemistry). View Reference
  3. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology: Fluorescence microscopy, Immunofluorescence). View Reference
  4. Takasaki Y, Kogure T, Takeuchi K, et al. Reactivity of anti-proliferating cell nuclear antigen (PCNA) murine monoclonal antibodies and human autoantibodies to the PCNA multiprotein complexes involved in cell proliferation. J Immunol. 2001; 166(7):4780-4787. (Biology: Western blot). View Reference
  5. Uckun FM, Tuel-Ahlgren L, Waddick KG, et al. Physical and functional interactions between Lyn and p34cdc2 kinases in irradiated human B-cell precursors. J Biol Chem. 1996; 271(11):6389-6397. (Biology). View Reference
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610037 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.