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Purified Mouse Anti-Cdk1/Cdc2 (pY15)
Purified Mouse Anti-Cdk1/Cdc2 (pY15)

Western blot analysis for Cdk1/Cdc2 (pY15).  Cell lysates were prepared from Saos-2 cells (Human osteosarcoma; ATCC HTB-85) and were either left untreated (lane 1) or treated (lane 2) with 50 µg/ml alkaline phosphatase for 30 minutes at 37°C. The top panel was probed with a mouse anti-Cdk1 antibody (Cat. No. 610037) and the bottom panel was probed with the mouse anti-Cdk1/Cdc2 (pY15) antibody at a 1:250 dilution with an expected band ~ 34 kDa.

Western blot analysis for Cdk1/Cdc2 (pY15).  Cell lysates were prepared from Saos-2 cells (Human osteosarcoma; ATCC HTB-85) and were either left untreated (lane 1) or treated (lane 2) with 50 µg/ml alkaline phosphatase for 30 minutes at 37°C. The top panel was probed with a mouse anti-Cdk1 antibody (Cat. No. 610037) and the bottom panel was probed with the mouse anti-Cdk1/Cdc2 (pY15) antibody at a 1:250 dilution with an expected band ~ 34 kDa.

Product Details
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BD Transduction Laboratories™
p34 [cdc2]
Human (QC Testing), Mouse, Rat (Predicted)
Mouse IgG1
Phosphorylated Human Cdc2 (pY15) Peptide
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
34 kDa
250 µg/ml
AB_399621
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612306 Rev. 1
Antibody Details
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44/Cdk1/Cdc2 (pY15)

Progression of the mammalian cell cycle is regulated by phosphorylation of many key proteins. Several classes of cyclins (A-E) act as regulatory subunits for cyclin-dependent kinases (cdks). Cdk1/Cdc2 (p34 [cdc2]) is the catalytic subunit of the maturation promoting factor (MPF), which includes the regulatory subunit cyclin B. During late S and G2 phase, cyclin B synthesis increases, allowing it to bind Cdc2. This begins the transition into M-phase of the mammalian cell cycle by initiating a series of phosphorylation and dephosphorylation events that lead to activation of the Cdc2/cyclin B complex. After binding to cyclin B, cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex during G2 phase. Phosphorylation of cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the end of G2 phase and completes activation of the cdc2/Cyclin B complex and facilitates entry into mitosis. During mitosis, cyclin B is targeted for degradation and Cdc2 becomes inactive again.

612306 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612306 Rev.1
Citations & References
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Development References (2)

  1. Borgne A, Meijer L. Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. J Biol Chem. 1996; 271(44):27847-27854. (Biology). View Reference
  2. Uckun FM, Tuel-Ahlgren L, Waddick KG, et al. Physical and functional interactions between Lyn and p34cdc2 kinases in irradiated human B-cell precursors. J Biol Chem. 1996; 271(11):6389-6397. (Biology). View Reference
612306 Rev. 1

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