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Purified Mouse Anti-B2 Bradykinin Receptor
Purified Mouse Anti-B2 Bradykinin Receptor

Western blot analysis of B2 Bradykinin Receptor on a rat pituitary lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1: 4000 dilution of the mouse anti-B2 Bradykinin Receptor antibody.

Purified Mouse Anti-B2 Bradykinin Receptor

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Purified Mouse Anti-B2 Bradykinin Receptor

Immunohistochemistry: Rat hippocampus, formalin-fixed paraffin-embedded tissue, with citrate pre-treatment (20X magnification).

Western blot analysis of B2 Bradykinin Receptor on a rat pituitary lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1: 4000 dilution of the mouse anti-B2 Bradykinin Receptor antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Immunohistochemistry: Rat hippocampus, formalin-fixed paraffin-embedded tissue, with citrate pre-treatment (20X magnification).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human (Tested in Development)
Mouse IgG2b, κ
Human B2 Bradykinin Receptor aa. 350-364
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
42 kDa
250 µg/ml
AB_397826
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
610452 Rev. 2
Antibody Details
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20/B2 Bradykinin Receptor

Bradykinin is a nine amino acid vasoactive peptide that elicits numerous physiologic responses such as vasodilation, smooth muscle spasm, and pain. Bradykinin is one of a family of such peptides called the kinins. The kinins are generated from high molecular weight precursors called kininogens from proteolysis induced by pathophysiologic conditions such as inflammation or allergy. The physiological actions of these kinins are mediated by their interaction with transmembrane receptors. There are two distinct bradykinin receptor subtypes: B1 and B2. Both are coupled to G-proteins. The B2 receptor subtype is found in healthy smooth muscle cells and neurons, whereas, the B1 receptors are only detected following tissue injury. The B2 receptor is similar in structure to other seven helix G-protein coupled receptors. Bradykinin has a relatively low affinity for B1 receptors, and interacts primarily with the B2 receptor.  This interaction stimulates several second messenger systems, including inositol phospholipid hydrolysis, arachidonic acid metabolism, tyrosine phosphorylation, and membrane depolarization and hyperpolarization.

610452 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610452 Rev.2
Citations & References
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Development References (5)

  1. Duchene J, Schanstra JP, Pecher C. A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation. J Biol Chem. 2002; 277(43):40375-40383. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Ewert S, Johansson B, Holm M, Helander HF, Fandriks L. The bradykinin BK2 receptor mediates angiotensin II receptor type 2 stimulated rat duodenal mucosal alkaline secretion. BMC Physiol. 2003; 3(1):1-9. (Biology: Immunohistochemistry, Western blot). View Reference
  3. Golser R, Gorren AC, Leber A. Interaction of endothelial and neuronal nitric-oxide synthases with the bradykinin B2 receptor. Binding of an inhibitory peptide to the oxygenase domain blocks uncoupled NADPH oxidation. J Biol Chem. 2000; 275(8):5291-5296. (Biology: Immunoprecipitation, Western blot). View Reference
  4. Powell SJ, Slynn G, Thomas C, Hopkins B, Briggs I, Graham A. Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14. Genomics. 1993; 15(2):435-438. (Biology). View Reference
  5. Xie P, Browning DD, Hay N, Mackman N, Ye RD. Activation of NF-kappa B by bradykinin through a Galpha(q)- and Gbeta gamma-dependent pathway that involves phosphoinositide 3-kinase and Akt. J Biol Chem. 2000; 275(32):24907-24914. (Biology: Western blot). View Reference
View All (5) View Less
610452 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.