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      Purified NA/LE Rat Anti-Mouse IL-6
      Product Details
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      BD Pharmingen™
      Il6; Il-6; Interleukin-6; B-cell hybridoma growth factor
      Mouse (QC Testing)
      Rat IgG1
      Mouse IL-6 Recombinant Protein
      ELISA Capture (Routinely Tested), Neutralization (Tested During Development)
      1.0 mg/ml
      15978,16193
      AB_2127480
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      ELISA:  Purified Rat Anti-Mouse IL-6 antibody (Cat. No. 554400 or 554398) can be useful as a capture antibody for sandwich ELISA measuring mouse IL-6 protein levels.  Purified Rat Anti-Mouse IL-6 antibody can be paired with Biotin Rat Anti-Mouse IL-6 antibody (Cat. No. 554402, clone MP5-32C11) as the detecting antibody, with recombinant mouse IL-6 (Cat. No. 554582) as the standard.  For testing mouse IL-6 in complex biological samples, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-6 ELISA Set (Cat. No 555240) or BD OptEIA™ Mouse IL-6 ELISA Kit (Cat. No. 550950).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554398 Rev. 2
      Antibody Details
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      MP5-20F3

      The MP5-20F3 monoclonal antibody specifically binds to mouse interleukin-6 (IL-6). The immunogen used to generate the MP5-20F3 hybridoma was recombinant mouse IL-6.

      554398 Rev. 2
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554398 Rev.2
      Citations & References
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      Development References (3)

      1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology: ELISA). View Reference
      2. Starnes HF Jr, Pearce MK, Tewari A, Yim JH, Zou JC, Abrams JS. Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-alpha challenge in mice. J Immunol. 1990; 145(12):4185-4191. (Biology). View Reference
      3. Suda T, O'Garra A, MacNeil I, Fischer M, Bond MW, Zlotnik A. Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas. Cell Immunol. 1990; 129(1):228-240. (Biology). View Reference
      554398 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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