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AEC Substrate Set

BD™ ELISPOT AEC Substrate Set

Product Details
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ELISPOT (Routinely Tested)


3-Amino-9-ethylcarbazole (AEC) is a substrate for peroxidase and is a widely-used chromogen for ELISPOT.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

No more than 15 minutes prior to use, mix one drop (20 µL) of AEC Chromogen with each 1 mL of AEC Substrate, vortex or mix. Discard any remaining prepared AEC Substrate after use.

Warning:  AEC Substrate (MN 51-2577KC) contains 7.99% methanol (w/w).

Hazard statements

Combustible liquid.

Harmful if swallowed.

May cause damage to organs.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth.

IF exposed or concerned: Call a POISON CENTER/doctor.

Store locked up. Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.

Danger: AEC Chromogen (MN 51-2578KC) contains 97.8% N,N-dimethylformamide (w/w).

Hazard statements

Flammable liquid and vapour.

Harmful if in contact with skin or inhaled.

Causes serious eye irritation.

May damage fertility or the unborn child.

Precautionary statements

Obtain special instructions before use. Do not handle until all safety precautions have been read and understood.

Avoid breathing dust/fume/gas/mist/vapors/spray.

Keep away from heat/sparks/open flames/hot surfaces. - No smoking. In case of fire: Use suitable extinguishing media to extinguish

Wear protective clothing / eye protection/ protective gloves.

Wash thoroughly after handling.

IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.  Continue rinsing. If eye irritation persists: Get medical advice/attention.

IF exposed or concerned: Get medical advice/attention

Dispose of contents/container in accordance with local/regional/national/international regulations.

Product Notices

  1. Please refer to for technical protocols.
551951 Rev. 4
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Description Quantity/Size Part Number EntrezGene ID
AEC Substrate 100 mL (1 ea) 51-2577KC N/A
AEC Chromogen 3 mL (1 ea) 51-2578KC N/A
551951 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.