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Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e)
Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e)

Effects of anti-Integrin α5 chain (CD49e) antibodies on K562 cell binding to fibronectin. Aliquots of K562 cell suspension, an erythroleukemia cell line expressing Integrin α5 chain (CD49e) receptor, were incubated in the presence of the designated concentrations of Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e) (Cat. No. 555614, • ) or Purified Mouse Anti-Human CD49e (Cat. No. 555651, □), for 30 minutes at room temperature. The suspensions were then transferred to plastic microtiter wells precoated with 12 µg/mL of fibronectin and incubated for 45 minutes at 37°C. Unattached cells were removed by aspiration and washing with PBS, and the attached cells were semi-quantitated using toluidine blue staining. The resulting absorbance signals are expressed relative to the average signal obtained without antibody.

Effects of anti-Integrin α5 chain (CD49e) antibodies on K562 cell binding to fibronectin. Aliquots of K562 cell suspension, an erythroleukemia cell line expressing Integrin α5 chain (CD49e) receptor, were incubated in the presence of the designated concentrations of Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e) (Cat. No. 555614, • ) or Purified Mouse Anti-Human CD49e (Cat. No. 555651, □), for 30 minutes at room temperature. The suspensions were then transferred to plastic microtiter wells precoated with 12 µg/mL of fibronectin and incubated for 45 minutes at 37°C. Unattached cells were removed by aspiration and washing with PBS, and the attached cells were semi-quantitated using toluidine blue staining. The resulting absorbance signals are expressed relative to the average signal obtained without antibody.

Product Details
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BD Pharmingen™
Integrin α5; Integrin alpha-5; ITGA5; ITA5; FNRA; VLA-5; VLA5A
Human (QC Testing)
Mouse RBF/ DnJ IgG1, κ
Human α5β1 Protein
Flow cytometry (Routinely Tested)
1.0 mg/ml
3678
AB_395981
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
555614 Rev. 12
Antibody Details
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IIA1

The IIA1 monoclonal antibody specifically recognizes CD49e, a type I transmembrane glycoprotein which is also known as Integrin α5 Chain or VLA-5 α chain (VLA-5α).  Following translation, CD49e is cleaved into two disulfide-linked chains of 135 and 25 kDa that associate with β1 integrin (CD29) to form the VLA-5 (Integrin α5/β1) complex. The VLA-5 complex is a well-established fibronectin receptor that is expressed on many cell types, including fibroblasts, endothelial cells, epithelial cells, platelets, thymocytes, T cells, B cells, the myeloid cell line U937, K562 and some melanoma cell lines. This clone inhibits α5β1 binding of ligands. It is useful for studies of the expression and function of this integrin.

555614 Rev. 12
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
555614 Rev.12
Citations & References
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Development References (1)

  1. Tran Van Nhieu G, Isberg RR. The Yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the alpha 5 beta 1 integrin receptor. J Biol Chem. 1991; 266(36):24367-24375. (Biology). View Reference
555614 Rev. 12

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.