Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
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The 5F/B9 monoclonal antibody specifically recognizes CD73 or Ecto-5'-nucleotidase (5'-NT), a 68-kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities. 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues. Membrane-bound and/or intracytoplasmic CD73 is found in a variety of organs and tissues, including liver, kidney, adrenal cortex, spleen, muscle, nervous tissue, and heart. The expression of CD73 on mouse leukocytes has been well characterized. In the rat, CD73 has been detected on subsets of peripheral B and T lymphocytes, NK cells, and granulocytes, as well as bone marrow myeloid cells and some CD4+CD8- thymocytes. The 5F/B9 mAb does not inhibit the enzymatic activity of 5'-NT. Although soluble or plate-bound 5F/B9 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with plate-bound anti-rat CD3 mAb G4.18.
Development References (6)
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Carvalho GS, Luzio JP, Siddle K, Coombs RR. Detection of ecto-5'-nucleotidase on rat B and T lymphocyte subpopulations using a monoclonal antibody in rosetting reactions. J Immunol Methods. 1987; 102(1):119-126. (Biology). View Reference
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Gandhi R, Le Hir M, Kaissling B. Immunolocalization of ecto-5'-nucleotidase in the kidney by a monoclonal antibody. Histochem J. 1990; 95(2):165-174. (Immunogen: Immunohistochemistry, Immunoprecipitation). View Reference
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Mlodzik K, Loffing J, Le Hir M, Kaissling B. Ecto-5'-nucleotidase is expressed by pericytes and fibroblasts in the rat heart. Histochem J. 1995; 103(3):227-236. (Biology). View Reference
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Yamashita Y, Hooker SW, Jiang H, et al. CD73 expression and fyn-dependent signaling on murine lymphocytes. Eur J Immunol. 1998; 28(10):2981-2990. (Biology). View Reference
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Zenker W, Rinne B, Bankoul S, Le Hir M, Kaissling B. 5'-nucleotidase in spinal meningeal compartments in the rat. An immuno and enzyme histochemical study. Histochem J. 1992; 98(2):135-139. (Biology). View Reference
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Zimmermann H. 5'-Nucleotidase: molecular structure and functional aspects. Biochem J. 1992; 285((Pt 2)):345-365. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.