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Expression of Ly-6G on bone-marrow leukocytes. C57BL/6 bone-marrow cell suspensions were stained with either PE Rat Anti-Mouse Ly-6G (Cat. No. 551461, open histograms) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, filled histograms), in the presence of Mouse Fc Block™ purified anti-mouse CD16/CD32 (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463), and leukocyte subsets were distinguished by their light-scatter profiles. The left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
BD Pharmingen™ PE Rat Anti-Mouse Ly-6G
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For flow cytometry of leukocyte suspensions, we recommend the use of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
Development References (1)
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Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.