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Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with BD Horizon™ PE-CF594 Mouse anti-Mouse Siglec-G (Cat. No. 563337) and Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of Siglec-G versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse anti-Mouse Siglec-G and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
BD Horizon™ PE-CF594 Mouse anti-Mouse Siglec-G
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The SH1 monoclonal antibody specifically binds to mouse Siglec-G (sialic acid binding immunoglobulin-like lectin G), also known as Siglec10. Siglec-G is a Type I transmembrane glycoprotein that belongs to the Immunoglobulin superfamily. Siglec-G functions as an adhesion molecule that mediates sialic-acid dependent binding to cells. Siglec-G is expressed on cells of the B cell lineage. Siglec-G is most highly expressed by pre-B cells and B1a cells within the B cell lineage and is not detectable on T cells. Siglec-G can inhibit B cell receptor-mediated calcium signaling when it is overexpressed. Mice that lack Siglec-G have significantly increased numbers of B1a cells that begins early in development and is B cell intrinsic. Siglec-G-deficient mice have higher titers of natural IgM antibodies than their normal counterparts. Mouse Siglec-G is the ortholog of human Siglec-10 (also known as, CD330).
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
Development References (4)
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Aizawa H, Zimmermann N, Carrigan PE, Lee JJ, Rothenberg ME, Bochner BS. Molecular analysis of human Siglec-8 orthologs relevant to mouse eosinophils: identification of mouse orthologs of Siglec-5 (mSiglec-F) and Siglec-10 (mSiglec-G). Genomics. 2003; 82(5):521-530. (Biology). View Reference
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Hoffmann A, Kerr S, Jellusova J, et al. Siglec-G is a B1 cell-inhibitory receptor that controls expansion and calcium signaling of the B1 cell population. Nat Immunol. 2007; 8(7):695-704. (Biology). View Reference
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Jellusova J, Düber S, Gückel E, et al. Siglec-G regulates B1 cell survival and selection. J Immunol. 2010; 185(6):3277-3284. (Biology). View Reference
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Nitschke L. CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 2009; 230(1):128-143. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.