MiCK-2 Mouse Cytokine Positive Control Cells(RUO)
Characteristic staining of MiCK-2 Positive Control Cells with anti-mouse IL-4, IL-10 and GM-CSF. MiCK-2 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control (PE-R3-34; Cat. No. 554685; Panel A), PE-rat anti-mouse IL-4 (PE-11B11, Cat. No. 554435; Panel B), PE-rat anti-mouse IL-10 (PE-JES5-16E3, Cat. No. 554467; Panel C) or PE-rat anti-mouse GM-CSF (PE-MP1-22E9, Cat. No. 554406; Panel D). Despite fixation and freezing, the side-and forward-scattered light signals for these control cells (Panel F) remain similar to those for freshly-prepared lymphoid cell preparations (data not shown). Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown in Panels A-D).
BD Pharmingen™ MiCK-2 Mouse Cytokine Positive Control Cells
MiCK-2 Mouse Cytokine Positive Control Cells
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