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      FITC Mouse Anti-Rat CD61
      Product Details
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      BD Pharmingen™
      Integrin β3 chain; Itgb3; Integrin beta-3
      Rat (QC Testing)
      Mouse BALB/c IgG1, κ
      Rat (Sprague-Dawley) bone cell suspensions
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_395611
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      Other applications: The purified format of the F11 clone has been reported to be useful in immunoprecipitation and IHC on frozen or zinc-fixed sections. Please note that these applications are not tested at BD Biosciences Pharmingen. This clone is also available in the no azide/low endotoxin format (NA/LE)  as Cat. No. 554950.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      561909 Rev. 2
      Antibody Details
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      F11

      The F11 monoclonal antibody specifically recognizes integrin β3 chain (CD61), which associates with the integrin αv chain (CD51), to form the vitronectin receptor found on endothelial cells, myeloid cells, and osteoclasts, and with the αIIb chain (CD41) on platelets and megakaryocytes.  Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor.  The F11 antibody reportedly blocks the in vitro attachment of osteoclasts to several ligands.  Weak F11 antibody reactivity with human osteoclasts, megakaryocytes, and platelets has also been reported.

      561909 Rev. 2
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      561909 Rev.2
      Citations & References
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      View product citations for antibody "561909" on CiteAb

      Development References (3)

      1. Helfrich MH, Nesbitt SA, Dorey EL, Horton MA. Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectin, via a beta 3 integrin. J Bone Miner Res. 1992; 7(3):335-343. (Clone-specific: Blocking). View Reference
      2. Helfrich MH, Nesbitt SA, Horton MA. Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3. J Bone Miner Res. 1992; 7(3):346-351. (Immunogen: Immunoprecipitation). View Reference
      3. Kieffer N, Phillips DR. Platelet membrane glycoproteins: functions in cellular interactions. Annu Rev Cell Biol. 1990; 6:329-357. (Biology). View Reference
      561909 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.