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FITC Mouse Anti-Human CD8
FITC Mouse Anti-Human CD8

Flow cytometric analysis of CD8 expression on peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histogram) or FITC Mouse Anti-Human CD8 (Cat. No. 551347; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899).The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable cells.

Flow cytometric analysis of CD8 expression on peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histogram) or FITC Mouse Anti-Human CD8 (Cat. No. 551347; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899).The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable cells.

Product Details
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BD Pharmingen™
CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
Human (QC Testing)
Mouse IgG2a, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
925
AB_394159
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
551347 Rev. 6
Antibody Details
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G42-8

The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8αα homodimer or CD8αβ heterodimer. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. G42-8 stains approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. This antibody is useful in detecting CD8+ T cells when using DimerX I reagents.

551347 Rev. 6
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
551347 Rev.6
Citations & References
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Development References (1)

  1. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
551347 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.