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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 605 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
Companion Products
The 767329 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 6 (Siglec-6) which is also known as Obesity-binding protein 1 (OB-BP1), CD33 antigen-like 1 (CD33L1), or CD327. Siglec-6 (CD327) is a type I transmembrane glycoprotein that is encoded by SIGLEC6 which belongs to the Sialic acid-binding Ig-like lectins (Siglecs) superfamily. Siglec-6 (CD327) has one N-terminal Ig-like V-set domain followed by two Ig-like C2-type domains in its extracellular region. Its intracellular region has an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as a signaling lymphocyte activating molecule (SLAM)-like motif that can serve as a docking site for SAP (SLAM-associated protein). This I-type lectin can also exist in a soluble form of the extracellular region that lacks the transmembrane and cytoplasmic regions. Siglec-6 (CD327) is expressed on placental trophoblasts and B cells and at low levels on neutrophils. It specifically recognizes sialyl Tn-containing molecules and binds to leptin and is involved in cellular interactions and signaling.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
Development References (3)
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Botting RA, Bertram KM, Baharlou H, et al. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes.. J Leukoc Biol. 2017; 101(6):1393-1403. (Clone-specific: Flow cytometry). View Reference
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Madge PD, Maggioni A, Pascolutti M, et al. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy.. Sci Rep. 2016; 6:36012. (Clone-specific: Flow cytometry). View Reference
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Takei Y, Sasaki S, Fujiwara T, Takahashi E, Muto T, Nakamura Y. Molecular cloning of a novel gene similar to myeloid antigen CD33 and its specific expression in placenta.. Cytogenet Cell Genet. 1997; 78(3-4):295-300. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.