Skip to main content Skip to navigation
BV480 Mouse Anti-Human CD263 (TRAIL-R3)
Product Details
Down Arrow Up Arrow


BD OptiBuild™
TRAIL-R3; TRAILR3; TRAIL Receptor 3; TNFRSF10C ; TR10C; DCR1; LIT; TRID
Human (Tested in Development)
Mouse BALB/c IgG1
Recombinant human TRAIL R3/Fc Protein
Flow cytometry (Qualified)
0.2 mg/ml
HLDA8
AB_2743638
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV480 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
746314 Rev. 1
Antibody Details
Down Arrow Up Arrow
B-D44

The B-D44 monoclonal antibody specifically recognizes CD263 which is also known as, TNF-related apoptosis-inducing ligand receptor 3 (TRAIL Receptor 3/TRAIL-R3), Decoy receptor 1 (DCR1), Lymphocyte inhibitor of TRAIL (LIT), or TRID. CD263 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the TNF Receptor Superfamily which includes other TRAIL (CD253/APO-2 Ligand) Receptors: CD261 (TRAIL-R1), CD262 (TRAIL-R2), and CD264 (TRAIL-R4). Unlike CD261 or CD262 that contain an intracellular signaling death domain, CD263 (and CD264) lacks a cytoplasmic death domain and is unable to transduce apoptotic signals upon binding TRAIL. CD263 thus serves as a decoy receptor for TRAIL. It functions as a negative regulator of apoptotic signaling by competing with CD261 and CD262 for the binding of TRAIL. Overexpression of CD263 can reportedly attenuate TRAIL-induced apoptosis.

The antibody was conjugated to BD Horizon™ BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set.  BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.

746314 Rev. 1
Format Details
Down Arrow Up Arrow
BV480
The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 will also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV480
Violet 405 nm
440 nm
479 nm
746314 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "746314" on CiteAb

Development References (2)

  1. Baritaki S, Katsman A, Chatterjee D, Yeung KC, Spandidos DA, Bonavida B. Regulation of tumor cell sensitivity to TRAIL-induced apoptosis by the metastatic suppressor Raf kinase inhibitor protein via Yin Yang 1 inhibition and death receptor 5 up-regulation. J Immunol. 2007; 179(8):5441-5453. View Reference
  2. Mérino D, Lalaoui N, Morizot A, Schneider P, Solary E, Micheau O. Differential inhibition of TRAIL-mediated DR5-DISC formation by decoy receptors 1 and 2. Mol Cell Biochem. 2006; 26(19):7046-7055. View Reference
746314 Rev. 1

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.