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BD Horizon™ BV421 Rat anti-Mouse CD371 (Clec12A)
Clone 5D3/CD371 (also known as 1/06-5D3) (RUO)
Multicolor flow cytometric analysis of CD371 (Clec12A) expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560143/560245), PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD49b (Cat. No. 562453) antibodies and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histograms) or BD Horizon BV421 Rat Anti-Mouse CD371 (Clec12A) antibody (Cat. No. 564795; solid line histograms).
Overlapping histograms showing CD371 expression (or Ig Isotype control staining) on either CD11c-high CD45R/B220- (Left Panel) or CD11c-intermediate CD45R/B220+ (Right Panel) cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD371 (Clec12A) expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560143/560245), PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD49b (Cat. No. 562453) antibodies and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histograms) or BD Horizon BV421 Rat Anti-Mouse CD371 (Clec12A) antibody (Cat. No. 564795; solid line histograms).
Overlapping histograms showing CD371 expression (or Ig Isotype control staining) on either CD11c-high CD45R/B220- (Left Panel) or CD11c-intermediate CD45R/B220+ (Right Panel) cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD371 (Clec12A) expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560143/560245), PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD49b (Cat. No. 562453) antibodies and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histograms) or BD Horizon BV421 Rat Anti-Mouse CD371 (Clec12A) antibody (Cat. No. 564795; solid line histograms).
Overlapping histograms showing CD371 expression (or Ig Isotype control staining) on either CD11c-high CD45R/B220- (Left Panel) or CD11c-intermediate CD45R/B220+ (Right Panel) cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD371 (Clec12A) expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560143/560245), PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD49b (Cat. No. 562453) antibodies and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histograms) or BD Horizon BV421 Rat Anti-Mouse CD371 (Clec12A) antibody (Cat. No. 564795; solid line histograms).
Overlapping histograms showing CD371 expression (or Ig Isotype control staining) on either CD11c-high CD45R/B220- (Left Panel) or CD11c-intermediate CD45R/B220+ (Right Panel) cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Horizon™
CD371; CL12A; CLL-1; KLRL1; MICL; Micl; D230024O04
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse Clec12A Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738955
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Cy is a trademark of GE Healthcare.
- CF™ is a trademark of Biotium, Inc.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
564795 Rev. 3
Antibody Details
5D3/CD371
The 5D3/CD371 (aka, 1/06-5D3) monoclonal antibody specifically binds to mouse CD371 which is encoded by Clec12A (C-type lectin domain family 12 member A). CD371 is also known as Myeloid inhibitory C-type lectin-like receptor (MICL), C-type lectin-like molecule 1 (CLL-1) or Dendritic cell-associated lectin 2 (DCAL-2). CD371 is a ~30 kDa type II transmembrane glycoprotein that possesses one extracellular C-type lectin domain and one cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). CD371 is expressed on plasmacytoid dendritic cells, subsets of myeloid dendritic cells, monocytes, macrophages and B cells. It reportedly serves as a negative regulator of granulocyte and monocyte function.
564795 Rev. 3
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
564795 Rev.3
Citations & References
View product citations for antibody "564795" on CiteAb
Development References (4)
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Lahoud MH, Proietto AI, Ahmet F, et al. The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses.. J Immunol. 2009; 182(12):7587-94. (Immunogen: Flow cytometry, Fluorescence microscopy, Functional assay, Immunofluorescence, In vivo exacerbation, Stimulation). View Reference
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Marshall AS, Willment JA, Lin HH, Williams DL, Gordon S, Brown GD. Identification and characterization of a novel human myeloid inhibitory C-type lectin-like receptor (MICL) that is predominantly expressed on granulocytes and monocytes.. J Biol Chem. 2004; 279(15):14792-802. (Biology). View Reference
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Marshall AS, Willment JA, Pyz E, et al. Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation.. Eur J Immunol. 2006; 36(8):2159-69. (Biology). View Reference
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van Rhenen A, van Dongen GA, Kelder A, et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination between normal and leukemic stem cells.. Blood. 2007; 110(7):2659-66. (Biology). View Reference
564795 Rev. 3
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.