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This is a suggested alternate SKU for 560875.
562738_IMAGE2.png

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

562738_IMAGE2.png

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

562738Image1.png

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

562738Image1.png

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

562738_IMAGE2.png 562738_IMAGE2.png 562738Image1.png 562738Image1.png Blue-Cap-Violet-Label.png

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

Multicolor flow cytometric analysis of mouse CD184 (CXCR4) expression on BALB/c thymocytes. BALB/c thymocytes were stained with APC Rat Anti-Mouse CD4 antibody (Cat. No. 553051) and with either BD Horizon™ BV421 Rat IgG2b Isotype Control (Cat. No. 562603, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD184 antibody (Cat. No. 562738, Right Panel). The two color fluorescent dot plots were derived from gated events based on the light scattering characteristics for viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

Product Details
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BD Horizon™
CXCR4, C-X-C chemokine receptor type 4; Fusin; LESTR; PB-CKR; Sdf1r
Mouse (QC Testing)
Rat IgG2b, κ
GST-NCXCR4 fusion protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2737757
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Brilliant Violet™ 421 is a trademark of Sirigen.
  4. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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562738 Rev. 2
Antibody Details
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2B11/CXCR4

The 2B11/CXCR4 monoclonal antibody specifically binds to mouse CD184, which is also known as the C-X-C Chemokine Receptor type 4 , CXCR4.  CXCR4 (previously known as Fusin and LESTR), is a seven-transmembrane, G-protein-coupled receptor. It is the specific receptor for the CXC chemokine, SDF-1/CXCL12.  Mouse CXCR4 shows 91% homology at the amino acid level with human CXCR4. CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes.  Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry.  The mouse Cxcr4 gene has been mapped to chromosome 1.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

562738 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
562738 Rev.2
Citations & References
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View product citations for antibody "562738" on CiteAb

Development References (9)

  1. Bleul CC, Farzan M, Choe H, et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996; 382(6594):829-833. (Biology). View Reference
  2. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Biology). View Reference
  3. Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996; 272(5263):872-877. (Biology). View Reference
  4. Forster R, Kremmer E, Schubel A, et al. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998; 160(3):1522-1531. (Immunogen: ELISA, Flow cytometry, Fluorescence microscopy, Functional assay, Inhibition, Western blot). View Reference
  5. Gupta SK, Lysko PG, Pillarisetti K, Ohlstein E, Stadel JM. Chemokine receptors in human endothelial cells. Functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines. J Biol Chem. 1998; 273(7):4282-4287. (Biology). View Reference
  6. Heesen M, Berman MA, Benson JD, Gerard C, Dorf ME. Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor. J Immunol. 1996; 157(12):5455-5460. (Biology). View Reference
  7. Hesselgesser J, Halks-Miller M, DelVecchio V, et al. CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons. Curr Biol. 1997; 7(2):112-121. (Biology). View Reference
  8. Oberlin E, Amara A, Bachelerie F, et al. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996; 382(6594):833-835. (Biology). View Reference
  9. Schabath R, Muller G, Schubel A, Kremmer E, Lipp M, Forster R. The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation. J Leukoc Biol. 1999; 66(6):996-1004. (Clone-specific: Flow cytometry, Fluorescence microscopy, Western blot). View Reference
View All (9) View Less
562738 Rev. 2

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.