Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Finland (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      BV421 Mouse Anti-Human CD144
      BV421 Mouse Anti-Human CD144
      Flow cytometric analysis of CD144 expression on Human Umbilical Vein Endothelial Cells (HUVECs). HUVECs were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438: dashed line histogram) or BD Horizon BV421 Mouse anti-Human CD144 antibody (Cat. No. 565670/565671; solid line histogram).  The fluorescence histogram showing CD144 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      BV421 Mouse Anti-Human CD144
      Flow cytometric analysis of CD144 expression on Human Umbilical Vein Endothelial Cells (HUVECs). HUVECs were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438: dashed line histogram) or BD Horizon BV421 Mouse anti-Human CD144 antibody (Cat. No. 565670/565671; solid line histogram).  The fluorescence histogram showing CD144 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
      Down Arrow Up Arrow


      BD Horizon™
      VE-cadherin; Cadherin-5; CDH5; Vascular endothelial cadherin
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested)
      5 µl
      VI E092
      1003
      AB_2744284
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      7. An isotype control should be used at the same concentration as the antibody of interest.
      Show More blue down arrow Show Less blue up arrow
      565670 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      55-7H1

      The 55-7H1 antibody monoclonal antibody specifically binds to a calcium-independent epitope on Cadherin-5, a member of the cadherin family of calcium-dependent adhesion molecules. Cadherin-5 is also known as CD144 or VE-Cadherin. CD144 is expressed on endothelial cells in vivo and in vitro. It may play a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium.

      The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

      565670 Rev. 1
      Format Details
      Down Arrow Up Arrow
      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      565670 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "565670" on CiteAb

      Development References (7)

      1. Breier G, Breviario F, Caveda L, et al. Molecular cloning and expression of murine vascular endothelial-cadherin in early stage development of cardiovascular system.. Blood. 1996; 87(2):630-41. (Biology). View Reference
      2. Lampugnani MG, Resnati M, Raiteri M, et al. A novel endothelial-specific membrane protein is a marker of cell-cell contacts.. J Cell Biol. 1992; 118(6):1511-22. (Biology). View Reference
      3. Limaye V, Li X, Hahn C, et al. Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members.. Blood. 2005; 105(8):3169-77. (Clone-specific: Flow cytometry). View Reference
      4. Litwin M, Clark K, Noack L, et al. Novel cytokine-independent induction of endothelial adhesion molecules regulated by platelet/endothelial cell adhesion molecule (CD31).. J Cell Biol. 1997; 139(1):219-28. (Clone-specific: Flow cytometry). View Reference
      5. Martin-Padura I, Lostaglio S, Dejana E. CD144 (VEcadherin) workshop panel report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:752-754.
      6. Mutin M, Dignat-George F, Sampol J. Immunologic phenotype of cultured endothelial cells: quantitative analysis of cell surface molecules.. Tissue Antigens. 1997; 50(5):449-58. (Clone-specific: Flow cytometry). View Reference
      7. Vincent PA, Xiao K, Buckley KM, Kowalczyk AP. VE-cadherin: adhesion at arm's length.. Am J Physiol, Cell Physiol. 2004; 286(5):C987-97. (Biology). View Reference
      View All (7) View Less
      565670 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.