Skip to main content Skip to navigation
Biotin Mouse Anti-Rat IgM
Product Details
Down Arrow Up Arrow

BD Pharmingen™
Rat (QC Testing)
Mouse IgG1, κ
Pooled Rat Ig
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For the sandwich rat IgM ELISA, biotinylated G53-238 mAb may be used for detection with purified G53-238 mAb (Cat. No. 553885) for capture.  Biotinylated G53-238 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC-conjugated G53-238 mAb (Cat. No. 553887). For immunohistochemical staining, we recommend the use of biotinylated G53-238 mAb in our special formulation for immunohistochemistry, Cat. No. 550330.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553886 Rev. 11
Antibody Details
Down Arrow Up Arrow

The G53-238 antibody reacts specifically with rat IgM monomers and pentamers. It does not react with other Ig isotypes. G53-238 antibody has not been shown to stimulate B-cell proliferation.

553886 Rev. 11
Format Details
Down Arrow Up Arrow
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553886 Rev.11
Citations & References
Down Arrow Up Arrow
View product citations for antibody "553886" on CiteAb
No Citations Found

No Citations are available for this Product

553886 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.