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Purified Mouse Anti-TNP
Product Details
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BD Pharmingen™
Mouse BALB/c IgG1, κ
TNP-keyhole limpet hemocyanin
ELISA (Routinely Tested), Isotype control (Tested During Development)
0.5 mg/ml
AB_395222
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

This immunoglobulin is useful for quantitation of TNP by the sandwich ELISA. For the sandwich TNP ELISA, mAb 107.3 is optimal for capture with anti-TNP 49.2 mAb (Cat. no. 554055) for detection.

Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservation agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
554054 Rev. 6
Antibody Details
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107.3

The 107.3 clone has an unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten not expressed on human or mouse cells. In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone 107.3 was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

This antibody is routinely tested by ELISA analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

554054 Rev. 6
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554054 Rev.6
Citations & References
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No Citations Found

No Citations are available for this Product

554054 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.