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PE Mouse Anti-Human CD8β
Product Details
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BD FastImmune™
CD8B; CD8B1; CD8 beta; CD8β; Leu2; Ly3; LYT3
Mouse BALB/c IgG2a, κ
CD8+ suppressor T-cell
Flow cytometry
6 μg/mL
20 μL

641057 Rev. 1
Antibody Details
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The CD8β antibody, clone 2ST8.5H7, is derived from the hybridization NS-1 mouse tumor cells with splenocytes from Balb/c mice immunized with CD8+ suppressor T-cell clone termed 20a6.

The CD8β antibody recognizes an epitope formed by the combination of CD8 alpha and beta chains. The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (32, 30 kilodaltons (kDa)), while CD8+CD16+ natural killer (NK) lymphocytes and CD8+ T-cell receptor (TCR)-γ/δ+ T lymphocytes express only an α/α homodimer. CD8β can therefore be used to selectively bind to CD8+ T cells while excluding CD8+ NK cells. The CD8 antigenic determinant binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex can play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3/TCR complex.

641057 Rev. 1
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
641057 Rev.1
Citations & References
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Development References (30)

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View All (30) View Less
641057 Rev. 1

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