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Anti-Human Control γ1 FITC/γ1 PE/CD3 PerCP

Anti-Human Control γ1 FITC/γ1 PE/CD3 PerCP

(RUO (GMP))
Product Details
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BD FastImmune™
Human
Controls
RUO (GMP)
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

Mouse γ1 (IgG1), clone X40, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood mononuclear cells (PBMCs).

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

Mouse γ1 (IgG1) recognizes a keyhole limpet hemocyanin (KLH) antigen not expressed on human cells.

CD3 recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kdaltons (kDa). The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).


Mouse γ1 (IgG1), clone X40, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood mononuclear cells (PBMCs).

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

Mouse γ1 (IgG1) recognizes a keyhole limpet hemocyanin (KLH) antigen not expressed on human cells.

CD3 recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kdaltons (kDa). The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).

Preparation And Storage

The FASTIMMUNE reagent is supplied as a combination of γ1 FITC, γ1 PE, and CD3 PerCP in 1.0 mL of phosphate-buffered saline containing BSA and 0.1% sodium azide. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340369 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
Mouse IgG1 Isotype Control FITC X40 IgG1, κ N/A
Mouse IgG1 Isotype Control PE L293 IgG1, κ N/A
CD3 PerCP SK7 IgG1, κ N/A
340369 Rev. 1
Citations & References
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Development References (11)

  1. Brenner M, Groh V, Porcelli A, et al. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.
  2. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  3. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
  4. Garson JA, Beverley PCL, Coakham HB, Harper EJ. Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982; 298:375-377. (Biology).
  5. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  6. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology).
  7. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
  8. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986; 137(8):2501-2507. (Biology). View Reference
  9. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  10. Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation.. Cytometry. 1995; 20(2):127-33. (Biology). View Reference
  11. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
View All (11) View Less
340369 Rev. 1

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