Lyse/Fix Buffer 5X(RUO)
Flow cytometric analysis of ERK1/2 (pT202/pY204) on human whole blood treated with BD Phosflow™ Lyse/Fix Buffer. Freshly isolated human whole blood was left either untreated (shaded histogram) or treated with 400 nM PMA at 37°C for 10 minutes (open histogram), then fixed with Lyse/Fix Buffer 5X (Cat. No. 558049). Leukocytes were spun down and washed once with Hank's solution, followed by permeabilization with BD Phosflow™ Perm Buffer II (Cat. No. 558052) for 30 minutes. After washing the cells twice with BD Pharmingen™ Stain Buffer (Cat. No. 554656554657), the cells were stained with Alexa Fluor® 488 mouse anti-ERK1/2 (pT202/pY204) (Cat. No. 612592). Fluorescence histograms depicting ERK1/2 (pT202/pY204) expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
BD™ Phosflow Lyse/Fix Buffer 5X
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