Skip to main content Skip to navigation
Biotin Rat Anti-Mouse CD51
Biotin Rat Anti-Mouse CD51

Flow cytometric analysis of CD51 on mouse bone marrow myeloid cells. BALB/c bone marrow leukocytes were either left unstained (left panel) or stained with Biotin Rat Anti-Mouse CD51 (Cat. No. 551380, right panel), followed by PE Streptavidin (Cat. No. 554061). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD51, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD51 positive (right panel). The contour plots were based on the gated events with the forward and side light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD FACScan™

Flow cytometric analysis of CD51 on mouse bone marrow myeloid cells. BALB/c bone marrow leukocytes were either left unstained (left panel) or stained with Biotin Rat Anti-Mouse CD51 (Cat. No. 551380, right panel), followed by PE Streptavidin (Cat. No. 554061). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD51, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD51 positive (right panel). The contour plots were based on the gated events with the forward and side light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD FACScan™

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Itgav; Integrin alpha-V; Integrin αV ; VNRA; Vitronectin receptor alpha
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Mouse (BALB/c) IL-2-activated killer (LAK) Cells
Flow cytometry (Routinely Tested)
0.5 mg/ml
16410
AB_394174
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Flow cytometry: Since this antigen is expressed at low density on the cell surface, it may be desirable to use a second-step reagent conjugated to a "bright" fluorochrome, such as PE Streptavidin (Cat. No. 554061).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
551380 Rev. 4
Antibody Details
Down Arrow Up Arrow
RMV-7

The RMV-7 monoclonal antibody specifically binds to CD51, the 140 kDa integrin αV chain. Heterodimers of CD51 with several integrin β chains function as receptors for extracellular matrix proteins. CD51/CD61 (αVβ3 integrin, vitronectin receptor) mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, von Willebrand factor, and CD31 (PECAM-1). CD51 is expressed on activated T lymphocytes, polymorphonuclear granulocytes, blastocysts, and osteoclasts. CD51 is reportedly not detectable on mouse platelets using either H9.2B8 or RMV-7 antibody clones. CD51 also forms heterodimers with CD29 (integrin β1), integrins β5, β6, and β8 chains. αV integrins have diverse functions in development and homeostasis. The RMV-7 antibody reportedly blocks LAK-cell binding to vitronectin, fibronectin, fibrinogen, and CD31. Furthermore, the RMV-7 antibody reportedly inhibits LAK-cell cytoxicity against certain target cells by interfering with the binding of LAK cells to their target cells.

551380 Rev. 4
Format Details
Down Arrow Up Arrow
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
551380 Rev.4
Citations & References
Down Arrow Up Arrow

Development References (8)

  1. Bader BL, Rayburn H, Crowley D, Hynes RO. Extensive vasculogenesis, angiogenesis, and organogenesis precede lethality in mice lacking all alpha v integrins. Cell. 1998; 95(4):507-519. (Biology). View Reference
  2. Frieser M, Hallmann R, Johansson S, Vestweber D, Goodman SL, Sorokin L. Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins. Eur J Immunol. 1996; 26(12):3127-3136. (Biology). View Reference
  3. Moulder K, Roberts K, Shevach EM, Coligan JE. The mouse vitronectin receptor is a T cell activation antigen. J Exp Med. 1991; 173(2):343-347. (Biology). View Reference
  4. Nakamura I, Pilkington MF, Lakkakorpi PT, et al. Role of alpha(v)beta(3) integrin in osteoclast migration and formation of the sealing zone. J Cell Sci. 1999; 112(22):3985-3993. (Biology). View Reference
  5. Narumiya S, Abe Y, Kita Y, et al. Pre-B cells adhere to fibronectin via interactions of integrin alpha 5/alpha V with RGDS as well as of integrin alpha 4 with two distinct V region sequences at its different binding sites. Int Immunol. 1994; 6(1):139-147. (Biology: Blocking). View Reference
  6. Piali L, Hammel P, Uherek C, et al. CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium. J Cell Biol. 1995; 130(2):451-460. (Biology: Blocking). View Reference
  7. Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor. J Biol Chem. 1995; 270(19):11522-11531. (Biology). View Reference
  8. Takahashi K, Nakamura T, Koyanagi M, et al. A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction. J Immunol. 1990; 145(12):4371-4379. (Immunogen: Blocking, Immunoprecipitation). View Reference
View All (8) View Less
551380 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.