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Overview
The BD Rhapsody™ ATAC-Seq Assays enable you to either generate highly reproducible open chromatin profiling data from single cells or perform a multiomic analysis of open chromatin accessibility and transcriptome of single cells in one experiment using the BD Rhapsody™ Single-Cell Analysis System.
Are you interested in learning more about multiomic epigenomic profiling? Watch our webinar to learn how to combine image-based spectral cell sorting with ATAC-seq and whole transcriptome assays to decipher gene regulation in immunology research.
Learn more from the BD Rhapsody™ ATAC-Seq Assay Brochure.
ATAC-Seq: Revolutionizing Single-Cell Multiomics with Ease and Speed
Aymeric Silvin, a researcher from IGR (Institut Gustave Roussy) tested our kit and praised its ease of use and quick setup. He highlighted the high-quality data generated, making it an invaluable tool for researchers. Don't miss out on this revolutionary product - watch a short snapshot of the video.
Why Choose BD Rhapsody™ ATAC-Seq?
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Unlock Chromatin Secrets: uncover a new layer of biology within chromatin accessibility patterns, gene regulation, and epigenetic dynamics - all on the same cell and with a wide range of cell inputs. -
Enhance cost Efficiency: the BD Rhapsody™ ATAC-Seq Assay has been designed to be cost-effective without compromising quality. -
Next-Level Insights: with our BD Rhapsody™ Single-Cell Multiomic ATAC-Seq Assay, you will reveal gene regulation networks with great sensitivity and specificity. -
Sample Tagging Enabled: compatibility with Custom BD® Nuclear Antibody-Oligonucleotide Conjugates allows to combine and simultaneously process multiple samples in one single reaction, further optimizing costs.
Features
Validated on the new BD Rhapsody™ Enhanced Cell Capture Beads V3, BD Rhapsody™ ATAC-Seq Assays generate consistent, high-quality data across different samples and users and a wide range of cell inputs. Key features include:
- High sensitivity and specificity across different experimental conditions
- Capable of accommodating a wide range of cell inputs
- Integrated epigenomic and transcriptomic characterization on the same cells
- Compatibility with Custom BD® Nuclear Antibody-Oligonucleotide Conjugates
Applications
More accurate and comprehensive picture of cellular identities with the BD Rhapsody™ Single-Cell Multiomic ATAC-Seq Assay
In this study, the BD Rhapsody™ Single-Cell Multiomic ATAC-Seq Assay was used to analyze 2,190 nuclei from human peripheral blood mononuclear cells (PBMCs). A joint whole transcriptome analysis (WTA) and ATAC-seq dimensionality reduction was performed by Uniform Manifold Approximation and Projection (UMAP) and cells were annotated as members of distinct cell types using the WTA data from a PBMC reference atlas.
Enrichment of cell type-specific transcription factor motifs in PBMCs
The heat map shows normalized enrichment scores of cell type–specific transcription factor motifs. Motif scores were calculated using a binomial distribution, determining the relative enrichment of each motif in differentially accessible regions of a given cell type compared to GC-matched background regions. The scores were then normalized across cell types per motif on a 0–1 scale, where 0 indicates least enrichment and 1 indicates highest enrichment of each motif.
Comparison between single-cell ATAC-seq data and WTA data in PBMCs
A. Read density across each ATAC-seq cluster at the transcription start sites of cell type marker genes.
B. Violin plots showing cell type-specific gene expression in WTA data.
Concordance between gene expression and inferred gene activity score
This heatmap shows Pearson’s correlation coefficients between ATAC-seq gene activity scores and gene expression values in PBMCs, with each row representing a cell type in WTA data and each column a cell type in ATAC-seq data.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.