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Purified Mouse Anti-Human CD61
Purified Mouse Anti-Human CD61

Western blot analysis of Integrin β3 on a human platelet lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of the anti- human CD61 antibody.

Western blot analysis of Integrin β3 on a human platelet lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of the anti- human CD61 antibody.

Product Details
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BD Transduction Laboratories™
Integrin β3 chain
Human (QC Testing)
Mouse IgG1
Human Integrin β3 aa. 592-712
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
104 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611140 Rev. 2
Antibody Details
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1/Integrin Beta 3 Chain

Integrins are heterodimeric transmembrane receptors that mediate cell-cell or cell-matrix adhesion. They contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. Although there is a high degree of redunancy, each integrin has a specific biological function. For example, the β3 subunit associates with αIIb in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. In endothelial cells (EC), β3 complexes with the αv subunit to form the vitronectin receptor. This receptor mediates endothelial cell adhesion to vitronectin, fibrinogen, von Willebrand factor, thrombospondin, laminin, and fibronectin. In confluent EC cultures, the αvβ3 integrin localizes to focal adhesions at the cell body and cell-cell borders. Thus, continued study of individual integrin subunits will provide insights to mechanisms of cell adhesion and signaling.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611140 Rev. 2
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611140 Rev.2
Citations & References
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Development References (5)

  1. Albelda SM, Daise M, Levine EM, Buck CA. Identification and characterization of cell-substratum adhesion receptors on cultured human endothelial cells. J Clin Invest. 1989; 83(6):1192-2002. (Biology). View Reference
  2. Chen YP, Djaffar I, Pidard D, et al. Ser-752-->Pro mutation in the cytoplasmic domain of integrin beta 3 subunit and defective activation of platelet integrin alpha IIb beta 3 (glycoprotein IIb-IIIa) in a variant of Glanzmann thrombasthenia. Proc Natl Acad Sci U S A. 1992; 89(21):10169-10173. (Biology). View Reference
  3. Greenwood JA, Theibert AB, Prestwich GD, Murphy-Ullrich JE. Restructuring of focal adhesion plaques by PI 3-kinase. Regulation by PtdIns (3,4,5)-p(3) binding to alpha-actinin. J Cell Biol. 2000; 150(3):627-642. (Biology: Immunoprecipitation). View Reference
  4. Tang S, Gao Y, Ware JA. Enhancement of endothelial cell migration and in vitro tube formation by TAP20, a novel beta 5 integrin-modulating, PKC theta-dependent protein. J Cell Biol. 1999; 147(5):1073-1084. (Biology: Western blot). View Reference
  5. Yip KP, Marsh DJ. An Arg-Gly-Asp peptide stimulates constriction in rat afferent arteriole. Am J Physiol. 1997; 273(5):F768-F776. (Biology: Immunohistochemistry). View Reference
View All (5) View Less
611140 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.