R718 Rat Anti-Mouse CD4
Clone RM4-5 (also known as RM4.5)(RUO)
Two-color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody (Cat. No. 553061/553062/561827) and either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; Left Plot) or BD Horizon™ R718 Rat Anti-Mouse CD4 (Cat. No. 566939/ 566940; Right Plot) at 0.125 µg/test. A two-parameter pseudocolor density plot showing the correlated expression of CD4 (or Ig Isotype control staining) versus CD3ε was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ R718 Rat Anti-Mouse CD4
Regulatory Status Legend
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