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Flow cytometric analysis of CD1d expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD1d (Cat. No. 550255/561757; solid line histogram) and PE Mouse IgG1, κ Isotype Control (Cat. No.555749; dashed line histogram). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
BD Pharmingen™ PE Mouse Anti-Human CD1d
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The CD1d42 monoclonal antibody recognizes CD1d. Cell surface CD1d is structurally homologous to Class I MHC molecules. It consists of a glycosylated type I transmembrane α chain (43-49 kDa) that is non-covalently associated with β2-microglobulin. CD1d is a member of the CD1 family of molecules, which belong to the immunoglobulin superfamily. Sequence homology data classifies the CD1 molecules into two groups. Group 1 includes CD1a, CD1b and CD1c molecules; group 2 includes CD1d molecules and their homologs in other species. CD1d is expressed on cortical thymocytes, B cells, dendritic cells, monocytes, and some nonlymphoid cells including intestinal epithelial cells, hepatocytes and keratinocytes. It is not expressed on resting mature T cells. Studies suggest that CD1d participates in lipid antigen presentation to CD1d-restricted NKT cells.
Development References (5)
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Exley M, Garcia J, Wilson SB, et al. CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes. Immunology. 2000; 100(1):37-47. (Biology). View Reference
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Hong S, Scherer DC, Singh N. Lipid antigen presentation in the immune system: lessons learned from CD1d knockout mice. Immunol Rev. 1999; 169:31-44. (Biology). View Reference
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Ronger-Savle S, Valladeau J, Claudy A, et al. TGFbeta inhibits CD1d expression on dendritic cells. J Invest Dermatol. 2005; 124(1):116-118. (Clone-specific: Flow cytometry). View Reference
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Somnay-Wadgaonkar K, Nusrat A, Kim HS. Immunolocalization of CD1d in human intestinal epithelial cells and identification of a beta2-microglobulin-associated form. 1999; 11(3):383-392. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.