Flow cytometric profile of CD134 expression on non-stimulated peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with either PE Mouse Anti-Human CD134 (Cat. No. 555838, solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram). The fluorescence histograms depicting CD134 (or Ig isotype control) expression were derived from gated events with the side and light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Flow cytometric profile of CD134 expression on PHA-stimulated peripheral blood lymphocytes. Human peripheral blood lymphocytes were stimulated with PHA and incubated with recombinant OX40 ligand (R&D Systems, Cat. No. 1054-ox), then stained with either PE Mouse Anti-Human CD134 (Cat. No. 555838, solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram). The fluorescence histograms depicting CD134 (or Ig isotype control) expression were derived from gated events with the side and light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Flow cytometric profile of CD134 expression on non-stimulated peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with either PE Mouse Anti-Human CD134 (Cat. No. 555838, solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram). The fluorescence histograms depicting CD134 (or Ig isotype control) expression were derived from gated events with the side and light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Flow cytometric profile of CD134 expression on PHA-stimulated peripheral blood lymphocytes. Human peripheral blood lymphocytes were stimulated with PHA and incubated with recombinant OX40 ligand (R&D Systems, Cat. No. 1054-ox), then stained with either PE Mouse Anti-Human CD134 (Cat. No. 555838, solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram). The fluorescence histograms depicting CD134 (or Ig isotype control) expression were derived from gated events with the side and light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.
Product Details
BD Pharmingen™
OX40; TNFRSF4; TXGP1L; OX40L Receptor
Human (QC Testing)
Mouse IgG1, κ
Human HUT 102
Flow cytometry (Routinely Tested)
20 µl
IV A107, V BP048, V A068, VI C-31
7293
AB_396161
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
PE Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555749
PE Mouse Anti-Human CD134 RUO
Size
25 Tests
Cat No.
561700
555838 Rev. 7
Antibody Details
ACT35
The ACT35 monoclonal antibody specifically binds to CD134 which is also known as OX40. The 35 kDa CD134 polypeptide is encoded by the TNFRSF4 gene. CD134 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. CD134 is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts. CD134 functions as a T cell costimulatory receptor when bound by OX40 Ligand/TNFSF4 that is expressed by antigen presenting cells. CD134 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells. Analysis of the nucleotide sequence of the human TNFRSF4 cDNA reveals strong homology with the rat Tnfrsf4 cDNA sequence. OX40 was clustered as CD134 in the Sixth International Workshop on Human Leukocyte Differentiation Antigens.
555838 Rev. 7
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
555838 Rev.7
Citations & References
View product citations for antibody "555838" on CiteAb
Development References (3)
-
Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
-
Latza U, Durkop H, Schnittger S, et al. The human OX40 homolog: cDNA structure, expression and chromosomal assignment of the ACT35 antigen. Eur J Immunol. 1994; 24(3):677-683. (Biology). View Reference
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
555838 Rev. 7
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.