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Flow cytometric analysis of CD108 expression on HPB-ALL cells. HPB-ALL cells were stained with either PE Mouse Anti-Human CD108 (Cat. No. 552830; solid line histogram) or PE Mouse IgG2a, κ Isotype Control (Cat. No. 555574; dashed line histogram). Fluorescence histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.
BD Pharmingen™ PE Mouse Anti-Human CD108
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The KS-2 monoclonal antibody specifically recognizes the John-Milton-Hagen (JMH) blood group antigen. CD108 is a 76-80 kDa glycosylphosphatidylinositol (GPI)-linked protein. It is expressed on erythrocytes, at low levels on lymphocytes, but higher levels are detected on lymphoblasts and lymphoblastic cell lines. An adhesion function for CD108 has been suggested but this has not been demonstrated. Autoantibodies to this molecule are not uncommon and often associated with the loss of JMH antigen expression on erythrocytes.
Development References (4)
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Angelisová P, Hilgert I, Horejsí V. Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins. Immunogenetics. 1994; 39(4):249-256. (Biology). View Reference
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Bobolis KA, Moulds JJ, Telen MJ. Isolation of the JMH antigen on a novel phosphatidylinositol-linked human membrane protein. Blood. 1992; 79(6):1574-1581. (Biology). View Reference
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Mudad R, Rao N, Angelisova P, Horejsi V, Telen MJ. Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion. 1996; 35(7):566-570. (Biology). View Reference
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Yamada A, Kubo K, Takeshita T, et al. Molecular cloning of a glycosylphosphatidylinositol-anchored molecule CDw108. J Immunol. 1999; 162(7):4094-4100. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.