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FITC Rat Anti-Rat RT1A[a,b,l]
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This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
Product Details
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BD Pharmingen™
Rat (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgM, κ
DA rat spleen and lymph-node cells
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559996 Rev. 10
Antibody Details
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The B5 antibody reacts with epitope S of the rat MHC Class I antigen, haplotypes RT1[a] (eg, ACI, DA, COP), RT1[b] (eg, BP, BUF), and RT1[l] (eg, F344, LEW).  B5 antibody does not react with RT1[c] (eg, AUG, PVG/c) or RT1[u] (eg, AO, LOU, WF).

559996 Rev. 10
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
559996 Rev.10
Citations & References
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Development References (2)

  1. Butcher GW. A list of monoclonal antibodies specific for alloantigens of the rat. J Immunogenet. 1987; 14(2-3):163-176. (Biology). View Reference
  2. Smith RN, Amsden A, Sudilovsky O, Coleman N, Margolies R. The alloantibody response in the allogeneically pregnant rat. IV. Analysis of the alloantibody specificities with monoclonal antibodies. J Immunol. 1986; 136(11):4063-4069. (Immunogen). View Reference
559996 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.