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      FITC Mouse Anti-Human CD90
      FITC Mouse Anti-Human CD90
      Flow cytometric analysis of CD90 expression on HEL cell line. HEL cells were stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram) or FITC Mouse Anti-Human CD90 (Cat. No. 555595/561969; solid line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable HEL cells.
      FITC Mouse Anti-Human CD90
      Flow cytometric analysis of CD90 expression on HEL cell line. HEL cells were stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram) or FITC Mouse Anti-Human CD90 (Cat. No. 555595/561969; solid line histogram). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable HEL cells.
      Product Details
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      BD Pharmingen™
      THY1; Thy-1 antigen; Thy-1 membrane glycoprotein
      Human (QC Testing), Rhesus, Cynomolgus, Baboon, Pig, Dog (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human HEL Cell Line
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      V M07, BP222; VI BP28, E046
      7070
      AB_395969
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555595 Rev. 15
      Antibody Details
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      5E10

      The 5E10 monoclonal antibody specifically binds to human CD90 which is also known as Thy-1. CD90 is a 25-35 kDa glycophosphatidylinositol-anchored membrane glycoprotein of the Ig superfamily that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. The anti-CD90 antibody binds to a subset of immature CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is highly enriched for cells capable of long-term culture. The anti-CD90 antibody is useful for enriching high proliferative potential colony-forming cells (HIPP-CFC) that are primative progenitor cells.

      555595 Rev. 15
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      555595 Rev.15
      Citations & References
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      View product citations for antibody "555595" on CiteAb

      Development References (5)

      1. Baum CM, Weissman IL, Tsukamoto AS, Buckle AM, Peault B. Isolation of a candidate human hematopoietic stem-cell population. Proc Natl Acad Sci U S A. 1992; 89(7):2804-2808. (Biology). View Reference
      2. Craig W, Kay R, Cutler RL, Lansdorp PM. Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med. 1993; 177(5):1331-1342. (Immunogen). View Reference
      3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      4. Lansdorp PM, Thomas TE. AP Gee, ed. Bone Marrow Processing and Purging. Boca Raton FL: CRC Press; 1991.
      5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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      555595 Rev. 15

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.