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Multiparameter flow cytometric analysis of CD157 expression on human peripheral blood leucocyte populations. Whole blood was stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; Left Plot) or FITC Mouse Anti-Human CD157 antibody (Cat. No. 567727; Right Plot). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of CD157 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
BD Pharmingen™ FITC Mouse Anti-Human CD157
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
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Companion Products
The SY/11B5 monoclonal antibody specifically binds to CD157 which is also known as BST-1 (Bone marrow stromal antigen 1), BP-3/IF-7, Mo5, ADP-ribosyl cyclase 2, and cADPr hydrolase 2. CD157 is a 40-46 kDa glycophosphatidylinositol-linked cell membrane glycoprotein. It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. CD157 is expressed as a homodimer by a variety of cell types including bone marrow stromal cells, granulocytes, monocytes, macrophages, dendritic cells, endothelial cells, and B and T cell progenitors. In addition to its ectoenzyme activities, CD157 reportedly functions as a receptor involved in neutrophil and monocyte adhesion, transendothelial migration and diapedesis and in tumor cell migration.
Development References (6)
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Deaglio S, Sposato P, Barisone E, et al. Analysis of human CD157 by means of murine antibodies. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:368-369.
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Horenstein AL, Sizzano F, Lusso R, et al. CD38 and CD157 ectoenzymes mark cell subsets in the human corneal limbus. Mol Med. 2009; 15(3-4):76-84. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Malavasi F, Deaglio S, Funaro A, et al. Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiol Rev. 2008; 88(3):841-886. (Biology). View Reference
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Ortolan E, Arisio R, Morone S, et al. Functional role and prognostic significance of CD157 in ovarian carcinoma. J Natl Cancer Inst. 2010; 102(15):1160-1177. (Clone-specific: Blocking, Functional assay, Inhibition, Western blot). View Reference
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Ortolan E, Vacca P, Capobianco A, et al. CD157, the Janus of CD38 but with a unique personality. Cell Biochem Funct. 2002; 20(4):309-322. (Biology). View Reference
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Quarona V, Zaccarello G, Chillemi A, et al. CD38 and CD157: a long journey from activation markers to multifunctional molecules. Cytometry B Clin Cytom. 2013; 84(4):207-217. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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