The HBF-719 antibody reacts with the B6 alloantigen of Ly-49F, an inhibitory receptor which is expressed on subsets of natural killer (NK) cells, NK-1.1+ (or DX5+) T lymphocytes (NK-T cells), and on a population of memory CD8+ T lymphocytes in C57BL/6 mice, 1 but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice.3 The Ly-49 family of NK-cell receptors are disulfide linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains (CRD). The Ly-49 family members are expressed independently, such that an individual NKor T-cell may display more than one class of Ly-49 receptor homodimers. Ly-49F has an ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) in its cytoplasmic tail. Therefore, it is probably an inhibitory receptor. It weakly binds to cells expressing the H-2d MHC class I alloantigens.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.