The SN2 monoclonal antibody specifically recognizes a 37-42 kDa membrane glycoprotein expressed on a subset of peripheral lymphocytes, monocytes, immature thymocytes and on most platelets. CD165, also known as gp37 or AD2, is expressed at low levels on most thymocytes and thymic epithelial cells. CD165 has been detected on islet cells of the pancreas, Bowman's capsule of the kidney and on central nervous system neurons. SN2 antigen has been reported to be identical, or very similar, to the T-cell acute lymphoblastic leukemia antigen, TALLA-1. CD165 has been reported to play a role in the adhesion between thymocytes and thymic epithelial cells during development.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.