The 1F11monoclonal antibody specifically binds to the P2X7 receptor. P2X7 is also known as the P2X7 Receptor (P2X7R), Purinergic receptor P2X ligand-gated ion channel 7 (P2RX7), P2X7 purinoceptor, P2Z receptor, or ATP receptor. P2X7 is differentially expressed on a wide variety of cell types including T cells, B cells, macrophages, dendritic cells, mast cells, neurons, microglia, and astrocytes. Functional P2X receptors exist as trimers. Each P2X7 monomer contains intracellular N and C termini, two transmembrane domains, and a large extra cellular loop. When present at relatively high levels, extracellular adenosine 5'-triphosphate (ATP) binds to the P2X7 receptor and opens a channel for extracellular cation (eg, Na+, K+, Ca++) influx into cells. The P2X7 receptor-mediated cation influx can cause membrane depolarization and Ca++ mediated signaling cascades that trigger cellular responses, eg, the production and/or release of proinflammatory mediators (eg, IL-1β or IL-18) by cells of the immune system. P2X7 is also implicated in mediating ATP-induced apoptosis of cells. The 1F11 antibody reportedly blocks ATP-mediated cellular activation through P2X7R.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.