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This is a suggested alternate SKU for 561638.
mCD49b_BV421_NK1-1_PE.png

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

mCD49b_BV421_NK1-1_PE.png

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

563063Image1.png

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

563063Image1.png

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

mCD49b_BV421_NK1-1_PE.png mCD49b_BV421_NK1-1_PE.png 563063Image1.png 563063Image1.png Blue-Cap-Violet-Label.png

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of CD49b expression on mouse splenocytes. Splenic leucocytes from a C57BL6/J mouse were stained with PE Mouse Anti-Mouse NK1.1 antibody (Cat. No. 553165/557391/561046) and either BD Horizon™ BV421 Rat IgM, κ Isotype Control (Cat. No. 562708, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD49b antibody (Cat. No. 563063, Right Panel). Two color flow cytometric dot plots showing the expression CD49b (or Ig Isotype control staining) versus NK1.1 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Horizon™
itga2; Integrin alpha-2; DX5; Pan NK cell marker; VLAA2; VLA-2 alpha chain
Mouse (QC Testing)
Rat LEW, also known as Lewis IgM, κ
Mouse (C57BL/6) NK1.1+ cells propagated with rIL-2
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2737983
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  8. Brilliant Violet™ 421 is a trademark of Sirigen.
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563063 Rev. 1
Antibody Details
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DX5

The rat anti-mouse CD49b monoclonal antibody (clone DX5) specifically binds to the integrin α2 chain (CD49b). CD49b is a 150 kDa transmembrane glycoprotein that non-covalently associates with CD29 (integrin β1) to form the integrin α2β1 complex known as VLA-2. The rat anti-mouse CD49b antibody (clone DX5) has been reported to identify the majority of NK cells and a small T-cell subpopulation in most mouse strains (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, DBA/1, DBA/2, SJL, SWR, 129/J, but not NOD). The DX5 antibody also recognizes platelets that express high levels of CD49b. Multiparameter flow cytometric analysis has demonstrated that most lymphocytes which express NK-1.1 (NKR-P1B and NKR-P1C), as detectable by mouse anti-mouse NK-1.1 antibody (clone PK136), also express the DX5 antigen. Small DX5+ NK-1.1- and DX5- NK-1.1+ cell subsets are found, especially among the CD3-positive cell population. Some CD49b+ NK cells have been reported to gradually lose reactivity with the rat anti-mouse CD49b antibody (clone DX5) when cultured in the presence of recombinant human IL-2. The resulting DX5-negative cells have weakened cytotoxic activity when compared to the remaining DX5+ cells. This indicates that the DX5 antibody distinguishes functional subsets of NK cells. No activation or blocking activity of the rat anti-mouse antibody (clone DX5) has been observed. Staining of splenic NK cells with this antibody reportedly can be blocked by hamster anti-mouse CD49b antibody (clone HMα2).

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421  can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421  conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

563063 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
563063 Rev.1
Citations & References
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View product citations for antibody "563063" on CiteAb

Development References (4)

  1. Abrams J. Personal Communication. .
  2. Arase H, Saito T, Phillips JH, Lanier LL. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol. 2001; 167(3):1141-1144. (Immunogen: Blocking, Cell separation, Cytotoxicity, Flow cytometry). View Reference
  3. Moore TA, von Freeden-Jeffry U, Murray R, Zlotnik A. Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice. J Immunol. 1996; 157(6):2366-2373. (Biology). View Reference
  4. Ortaldo JR, Winkler-Pickett R, Mason AT, Mason LH. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J Immunol. 1998; 160(1):1158-1165. (Biology). View Reference
View All (4) View Less
563063 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.