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Flow cytometric analysis of CD62P expression on mouse platelets. Mouse platelets were either not activated (Left Panel) or activated with human thrombin (Sigma T-8885; 20 U/ml final concentration; 5 min at 37°C; Right Panel). The platelets were stained with either Alexa Fluor® 647 Rat IgG1, λ Isotype Control (Cat. No. 563713; dashed line histograms) or Alexa Fluor® 647 Rat Anti-Mouse CD62P antibody (Cat. No. 563674; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.


BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse CD62P

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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The RB40.34 monoclonal antibody specifically binds to mouse P-selectin (CD62P), a 140 kDa protein which is expressed on activated platelets, activated endothelial cells, and megakaryocytes. P-selectin mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells, mediates leukocyte rolling, and is involved in the migration of leukocytes into inflamed tissues. CD24 and CD162 (PSGL-1) are ligands of CD62P. mAb RB40.34 can block mouse P-selectin binding to its ligands in vitro and in vivo.
Development References (9)
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Aigner S, Ruppert M, Hubbe M, et al. Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin. Int Immunol. 1995; 7(10):1557-1565. (Biology). View Reference
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Austrup F, Vestweber D, Borges E, et al. P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues. Nature. 1997; 385(6611):81-83. (Clone-specific: Blocking). View Reference
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Bosse R, Vestweber D. Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum. Eur J Immunol. 1994; 24(12):3019-3024. (Immunogen: Blocking, ELISA, Flow cytometry, Immunoprecipitation). View Reference
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Hirata T, Furie BC, Furie B. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol. 2002; 169(8):4307-4313. (Clone-specific: Blocking). View Reference
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Katakai T, Mori KJ, Masuda T, Shimizu A. Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis. Int Immunol. 2002; 14(2):167-175. (Clone-specific: Immunohistochemistry). View Reference
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Ley K, Bullard DC, Arbones ML, et al. Sequential contribution of L- and P-selectin to leukocyte rolling in vivo. J Exp Med. 1995; 181(2):669-675. (Clone-specific: Blocking). View Reference
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Pendl GG, Robert C, Steinert M, et al. Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1. Blood. 2002; 99(3):946-956. (Clone-specific: Blocking). View Reference
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Tietz W, Allemand Y, Borges E, et al. CD4+ T cells migrate into inflamed skin only if they express ligands for E- and P-selectin. J Immunol. 1998; 161(2):963-970. (Clone-specific: Blocking). View Reference
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Yang J, Galipeau J, Kozak CA, Furie BC, Furie B. Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor. Blood. 1996; 87(10):4176-4186. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.