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Alexa Fluor® 647 Mouse Anti-Human IREM-2 (CD300e)
Alexa Fluor® 647 Mouse Anti-Human IREM-2 (CD300e)

Multiparameter flow cytometric analysis of IREM-2 (CD300e) expression on human peripheral blood leucocytes. Whole blood cells were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human IREM-2 (CD300e) antibody (Cat. No. 566315; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of IREM-2 (CD300e) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Multiparameter flow cytometric analysis of IREM-2 (CD300e) expression on human peripheral blood leucocytes. Whole blood cells were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human IREM-2 (CD300e) antibody (Cat. No. 566315; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of IREM-2 (CD300e) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
IREM2; CD300E; CD300LE; CLM2; CLM-2; CMRF35-A5; PIgR-2; LMIR6
Human (QC Testing)
Mouse IgG2a, κ
Human IREM-2 Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
AB_2739677
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566315 Rev. 1
Antibody Details
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UP-H1

The UP-H1 monoclonal antibody recognizes Immune receptor expressed by myeloid cells 2 (IREM-2) which is also known as CD300 antigen like family member E (CD300e), CMRF35-like molecule 2 (CLM2), or Polymeric immunoglobulin receptor 2 (PIgR2). IREM-2 is a 34 kDa type I transmembrane glycoprotein which belongs to the CMFRF35 subfamily of the immunoglobulin (Ig) superfamily. IREM-2 is expressed on monocytes, macrophages and myeloid dendritic cells (mDCs), but not on lymphoid dendritic cells or granulocytes. IREM-2 associates with DAP-12, an ITAM-containing adapter molecule, which provides activating signals. Crosslinking of IREM-2 leads to intracellular calcium mobilization, release of proinflammatory cytokines such as TNF, and increased expression of activation markers on monocytes and mDCs. IREM-2 activation of mDCs can reportedly enhance the alloreactive response of naive T cells.

        

566315 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566315 Rev.1
Citations & References
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Development References (3)

  1. Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M. Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004; 173(11):6703-6711. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
  2. Borrego F. The CD300 molecules: an emerging family of regulators of the immune system. Blood. 2013; 121(11):1951-1960. (Biology). View Reference
  3. Brckalo T, Calzetti F, Pérez-Cabezas B, Borràs FE, Cassatella MA, López-Botet M. Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells.. Eur J Immunol. 2010; 40(3):722-32. (Clone-specific: Flow cytometry). View Reference
566315 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.