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Flow cytometric analysis of CCL17 expression in mature human monocyte-derived dendritic cells. Monocyte-derived dendritic cells were generated from peripheral blood monocytes that were cultured (5 days) in complete RPMI medium containing recombinant human GM-CSF (Cat. No.550068) and IL-4 (Cat. No. 554605) proteins and restimulated (2 days) with lipopolysaccharide. The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714), and then stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CCL17 antibody (Cat. No. 566621; solid line histogram) at 0.5 ug/test. The histogram showing expression of human CCL17 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocyte-derived dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on the Technical Data Sheet are not lot specific.


BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CCL17

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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The T48-854 monoclonal antibody reacts with C-C motif chemokine 17 (CCL17), which is also known as Thymus and Activation-Regulated Chemokine (TARC), or Small-inducible cytokine A17 (SCYA17). CCL17 is produced by activated T cells, monocytes, or dendritic cells. It is constitutively produced by thymic stromal cells. CCL17 binds to and signals through cell surface C-C chemokine receptor type 4 (CCR4). CCR4 is expressed on NK cells, basophils, macrophages, and T cells, such as, Th2-like cells, skin-homing T cells, and some regulatory T cells. CCL17 plays a role in T cell development and is involved in the activation and trafficking of leucocytes, including effector T cells which mediate peripheral immune responses.
Development References (3)
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Bernardini G, Hedrick J, Sozzani S. Identification of the CC chemokines TARC and macrophage inflammatory protein-1 beta as novel functional ligands for the CCR8 receptor. J Immunol. 1998; 28(2):582-588. (Biology). View Reference
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D'Ambrosio D, Iellem A, Bonecchi R, et al. Selective up-regulation of chemokine receptors CCR4 and CCR8 upon activation of polarized human type 2 Th cells.. J Immunol. 1998; 161(10):5111-5. (Biology). View Reference
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Imai T, Yoshida T, Baba M, Nishimura M, Kakizaki M, Yoshie O. Molecular cloning of a novel T cell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector.. J Biol Chem. 1996; 271(35):21514-21. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.