Alexa Fluor® 488 Mouse anti-Sox2

BD Pharmingen™ Alexa Fluor® 488 Mouse anti-Sox2

Name: Alexa Fluor® 488 Mouse anti-Sox2
Brand: Alexa Fluor® 488 Mouse anti-Sox2
SKU: 560301

Clone 245610 (RUO)

Flow cytometric analysis of Sox2 expression on H9 cells. H9 human embryonic stem (ES) cells (WiCell, Madison, WI) were fixed with Fixation Buffer (Cat. No. 554655) for 10 minutes at 37°C, permeabilized with Perm Buffer III (Cat. No. 558050) on ice for 30 minutes, and then stained with either Alexa Fluor® 488 Mouse anti-Sox2 (Cat. No. 560301; solid line) or Alexa Fluor® 488 Mouse IgG2a Isotype Control (Cat. No.558055; dashed line). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable H9 stem cells. Flow cytometry was performed on a BD FACSCanto™ II.

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Product Details

Brand: BD Pharmingen™

Alternative Name: ANOP3, MCOPS3, MGC2413

Reactivity: Human (QC Testing), Mouse (Reactivity Confirmed in Development)

Isotype: Mouse IgG2a

Immunogen: Human SOX2 Recombinant Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_1645308

Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Recommended Assay Procedures

Any of the three BD™ Phosflow permeabilization buffers may be used with this antibody: BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885), Perm Buffer II (Cat. No. 558052), or Perm Buffer III (Cat. No. 558050).

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Fixation Buffer RUO

Size 100 mL Cat No. 554655

Perm Buffer III RUO

Size 125 mL Cat No. 558050

Alexa Fluor®488 Mouse IgG2a, κ Isotype control RUO

Size 50 Tests Cat No. 558055

560301 Rev. 2

Antibody Details

245610

The monoclonal antibody 245610 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. The 245610 antibody recognizes both human and mouse proteins. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain.

560301 Rev. 2

Format Details

Alexa Fluor™ 488

Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 488

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 517 nm

560301 Rev.2

Citations & References

View product citations for antibody "560301" on CiteAb

Development References (3)

Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005; 122:947-956. (Biology). View Reference
Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). View Reference
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-676. (Biology). View Reference

560301 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.