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BD FastImmune™ Antibody Cocktail (TNF-α FITC, CD69 PE, CD4 PerCP-Cy™5.5)
(RUO (GMP))Antibody Cocktail (TNF-α FITC, CD69 PE, CD4 PerCP-Cy™5.5)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Description
The Anti-Hu–TNF-α antibody, clone 6401.1111, is derived from a fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human TNF-α. The CD69 antibody, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line. The CD4 antibody, clone SK3, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes. The Anti-Human Tumor Necrosis Factor-α (Anti-Hu–TNF-α) antibody recognizes a 26- kilodalton (kDa) transmembrane protein. The CD69 antibody recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges. The CD4 antigen, with a molecular weight of 55 kDa, is present on Thelper/ inducer lymphocytes and monocytes.
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
Description | Clone | Isotype | EntrezGene ID |
---|---|---|---|
TNF FITC | 6401.1111 | IgG1, | N/A |
CD69 PE | 2D7/Basophils | IgG1, κ | N/A |
CD4 PerCP-CY5.5 | SK3 | IgG1, κ | N/A |
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