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BD Transduction Laboratories™ Purified Mouse Anti-Ran
Clone 20/Ran (RUO)
Western blot analysis of Ran on A431 cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Ran antibody.
Immunofluorescent staining of HeLa cells.
Western blot analysis of Ran on A431 cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Ran antibody.
Immunofluorescent staining of HeLa cells.
Product Details
BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Human Ran aa. 7-171
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
25 kDa
250 µg/ml
AB_397731
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610341 Rev. 2
Antibody Details
20/Ran
Ran is a highly conserved GTPase that is ubiquitously expressed. At steady-state, 80-90% of cellular Ran is located in the nucleus, with the remainder in the cytoplasm. The human TC4/Ran cDNA was originally isolated based on the predicted homology of its encoded protein product to Ras. Unlike other members of the Ras superfamily, Ran does not appear to contain signals for lipid modification, nor does it appear to show membrane localization. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 28kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1.
610341 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610341 Rev.2
Citations & References
View product citations for antibody "610341" on CiteAb
Development References (5)
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Drivas GT, Shih A, Coutavas E, Rush MG, D'Eustachio P. Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line. Mol Cell Biol. 1990; 10(4):1793-1798. (Biology). View Reference
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Faleiro L, Lazebnik Y. Caspases disrupt the nuclear-cytoplasmic barrier. J Cell Biol. 2000; 151(5):951-959. (Clone-specific: Immunofluorescence). View Reference
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Iborra FJ, Jackson DA, Cook PR. The path of RNA through nuclear pores: apparent entry from the sides into specialized pores. J Cell Sci. 2000; 113(2):291-302. (Clone-specific: Electron microscopy). View Reference
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Nemergut ME, Lindsay ME, Brownawell AM, Macara IG. Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor. J Biol Chem. 2002; 277(20):17385-17388. (Clone-specific: Western blot). View Reference
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Schwoebel ED, Ho TH, Moore MS. The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion. J Cell Biol. 2002; 157(6):963-974. (Clone-specific: Immunofluorescence, Western blot). View Reference
610341 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.