BD Rhapsody™ Scanner

A visual workflow QC system that can be combined with a microwell-based single-cell partitioning system.

Overview

The BD Rhapsody™ Scanner is designed to visualize all steps in the single-cell capture workflow and provide detailed analysis metrics at every step, enabling the user to make key decisions throughout the workflow. The scanner has been redesigned to be compatible for use with the BD Rhapsody™ HT Xpress System and BD Rhapsody™ 8-Lane Cartridge for higher-throughput applications, enabling a multisample workflow. It can also be used in combination with the BD Rhapsody™ Express System and BD Rhapsody™ Single-Lane Cartridge for lower-throughput needs.

Get more information from the BD Rhapsody™ System brochure.

FEATURES

The BD Rhapsody™ Scanner provides the ability to visualize single-cell capture and provides key quality metrics at every step

The BD Rhapsody^™ Scanner enables visual inspection of the cartridge and of each microwell through an optically clear window on the BD Rhapsody^™Single-Lane or BD Rhapsody^™ 8-Lane Cartridge

The BD Rhapsody™ Scanner provides the ability to visualize single-cell capture and provides key quality metrics at every step

The BD Rhapsody^™Scanner provides quality control measures such as cell capture rate and cell multiplet rates by direct imaging of the cartridge to analyze the quality of the cell preparation. Quality control metrics provide an indication if errors have occurred during the cartridge workflow allowing you to decide whether to proceed to sequencing.

The BD Rhapsody™ Scanner provides the ability to visualize single-cell capture and provides key quality metrics at every step

The viability of the input sample and success of each step of the cartridge workflow can be confirmed, giving the user the power to decide whether to change course or troubleshoot, if necessary, before expensive downstream sequencing

Multiomic Analysis of Treg heterogeneity for optimization of Treg-based cell therapies

BD Biosciences has launched this webinar, presenting BD Rhapsody^™ single cell isolation and preparation technology for proteomic and transcriptomic study of immune cells.

CD4+ regulatory T cells expressing FOXP3 (Tregs) play an essential role in maintaining self-tolerance. Treg cells play a key role in preventing autoimmune diseases and stimulating tolerance to allogeneic transplants. The use of Treg cells represents a promising innovative autoimmune disease and transplantation therapeutic strategy.

Treg cells are functionally and phenotypically heterogeneous when isolated from peripheral blood or after expansion for therapeutic purposes. The use of single-cell RNA sequencing allows us to analyze their transcriptome in detail. This allows us to identify different subgroups of Treg cells in their natural state and after expansion.

Prof. Makoto Miyara, MD, PhD is a Full Professor in Immunology. He is the coordinator of the reference laboratory for LED and antiphospholipid antibody syndrome at the APHP, Sorbonne-Université, Pitié-Salpêtrière. His specialty as a professional is to develop innovative diagnostic techniques, including AI use for pattern recognition and gene editing to detect autoantibodies. He completed his clinical training at Paris' Pitié-Salpêtrière hospital as an Internist. He leads a research group that focuses on manipulating tolerance mechanisms, primarily regulatory T cells, to offer innovative therapeutic strategies to promote allograft tolerance in transplants, restore immune homeostasis in autoimmune diseases, promote tissue regeneration or enhance antitumor immune responses. His main articles have been quoted more than 1500 times. He is the Lead Investigator of three ongoing Phase I clinical trials evaluating Treg cell therapy in liver, kidney and lung transplants.