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BD Transduction Laboratories™ Purified Mouse Anti-Mouse Pericentrin
Clone 30/Pericentrin (RUO)
Western blot analysis of pericentrin expression on NIH-3T3 cell line lysate (Left Panel). Lysate was incubated with Purified Mouse Anti-Mouse Pericentrin (Cat. No. 611814/611815) for 2 hours at room temperature at 2, 1, 0.5, and 0.25 ug/ml (lanes 1-4, respectively). Pericentrin expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002) at a dilution of 1:2000.
Immunofluorescent staining on NIH-3T3 cells (Right Panel). Cells were incubated with Purified Mouse Anti-Mouse Pericentrin and pericentrin expression was visualized with FITC Goat Anti-Mouse Ig (Cat. No. 554001).
Western blot analysis of pericentrin expression on NIH-3T3 cell line lysate (Left Panel). Lysate was incubated with Purified Mouse Anti-Mouse Pericentrin (Cat. No. 611814/611815) for 2 hours at room temperature at 2, 1, 0.5, and 0.25 ug/ml (lanes 1-4, respectively). Pericentrin expression was visualized with HRP Goat Anti-Mouse Ig (Cat. No. 554002) at a dilution of 1:2000.
Immunofluorescent staining on NIH-3T3 cells (Right Panel). Cells were incubated with Purified Mouse Anti-Mouse Pericentrin and pericentrin expression was visualized with FITC Goat Anti-Mouse Ig (Cat. No. 554001).
Product Details
BD Transduction Laboratories™
Mouse (QC Testing)
Mouse IgG1
Mouse pericentrin aa. 1692-1814
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
220 kDa
250 µg/ml
AB_399294
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611815 Rev. 2
Antibody Details
30/Pericentrin
Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple α-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3 MDa-complex that includes γ-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and γ-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and γ-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity.
611815 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611815 Rev.2
Citations & References
View product citations for antibody "611815" on CiteAb
Development References (2)
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Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M. Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Cell. 1994; 76(4):639-650. (Biology). View Reference
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Young A, Dictenberg JB, Purohit A, Tuft R, Doxsey SJ. Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes. Mol Biol Cell. 2000; 11(6):2047-2056. (Biology). View Reference
611815 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.