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BD Transduction Laboratories™ Purified Mouse Anti- Hic5
Clone 34/Hic-5 (RUO)
Western blot analysis of Hic-5 on a rat lung lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Hic-5 antibody.
Immunofluorescence staining of human fibroblasts.
Western blot analysis of Hic-5 on a rat lung lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Hic-5 antibody.
Immunofluorescence staining of human fibroblasts.
Product Details
BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Mouse Hic-5 aa. 73-186
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
50 kDa
250 µg/ml
AB_398703
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
HRP Goat Anti-Mouse Ig RUO
Size
1 ML
Cat No.
554002
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
611165 Rev. 1
Antibody Details
34/Hic-5
Focal adhesions are cell structures terminal to the actin-stress fiber bundles. They attach cultured cells to their substratum or extracellular matrix. These adhesions occur through the integrin receptor molecules which link cytoskeletal proteins and extracellular matrix. The intracellular adhesion is composed of a number of proteins such as paxillin, VASP, vinculin, and the focal-adhesion kinases FAK and PYK2/CAKβ. Hic-5, also described as hydrogen peroxide inducible-mRNA, is a focal adhesion protein that binds to FAK and PYK2. It is ubiquitously expressed with the highest levels found in lung, spleen, and heart. Induction of Hic-5 is accomplished by hydrogen peroxide or TGFβ1 and is repressed in K-ras transformed cells. Like paxillin, Hic-5 is tyrosine phosphorylated in Src-transformed cells and is highly similar to paxillin in its primary structure. Both proteins contain LIM domains and LD motifs. In addition, Hic-5 localizes to focal adhesions and co-immunoprecipitates with PYK2/CAKβ in vivo. Thus, Hic-5 may be a substrate for CAKβ and play a role in signal transduction during proliferation, cell motility, and adhesion.
611165 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611165 Rev.1
Citations & References
View product citations for antibody "611165" on CiteAb
Development References (4)
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Jia Y, Ransom RF, Shibanuma M, Liu C, Welsh MJ, Smoyer WE. Identification and characterization of hic-5/ARA55 as an hsp27 binding protein. J Biol Chem. 2001; 276(43):39911-39918. (Biology: Immunoprecipitation, Western blot). View Reference
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Matsuya M, Sasaki H, Aoto H, et al. Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions. J Biol Chem. 1998; 273(2):1003-1014. (Biology). View Reference
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Shibanuma M, Mashimo J, Kuroki T, Nose K. Characterization of the TGF beta 1-inducible hic-5 gene that encodes a putative novel zinc finger protein and its possible involvement in cellular senescence. J Biol Chem. 1994; 269(43):26767-26774. (Biology). View Reference
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Wang X, Yang Y, Guo X, et al. Suppression of androgen receptor transactivation by Pyk2 via interaction and phosphorylation of the ARA55 coregulator. J Biol Chem. 2002; 277(18):15426-15431. (Biology: Immunoprecipitation, Western blot). View Reference
611165 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.