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BD Transduction Laboratories™ Purified Mouse Anti-GBF1
Clone 25/GBF1 (RUO)
Western blot analysis of GBF1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-GBF1 antibody.
Immunofluorescence staining of BC3H1 cells (Mouse brain smooth muscle-like cells; ATCC CRL-1443).
Western blot analysis of GBF1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-GBF1 antibody.
Immunofluorescence staining of BC3H1 cells (Mouse brain smooth muscle-like cells; ATCC CRL-1443).
Product Details
BD Transduction Laboratories™
Golgi-specific Brefeldin A (BFA)-resistance Factor 1
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human GBF1 aa. 1266-1379
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
206 kDa
250 µg/ml
AB_399487
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
HRP Goat Anti-Mouse Ig RUO
Size
1 ML
Cat No.
554002
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
612116 Rev. 1
Antibody Details
25/GBF1
The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgi-specific Brefeldin A (BFA)-resistance Factor (GBF1) is a guanine nucleotide exchange factor that contains a centrally located Sec7 domain, and a proline-rich region at the C-terminus. The Sec7 domain is commonly found in a variety of secretory proteins, and is required for activation of ARFs. GBF1 mRNA is ubiquitously expressed, and GBF1 protein localizes to the Golgi, as well as to perinuclear structures that contain COPI. BFA is a fungal heterocyclic lactone that disrupts membrane recruitment of ARFs. This action blocks protein secretion, and changes the morphology of various organelles, such as the Golgi. Overexpression of GBF1 suppresses BFA-induced changes in Golgi morphology, ARF activation, and coat protein recruitment. GBF1 interacts preferentially with ARF5 in the presence of Mg2+. Thus, GBF1 is a Golgi-specific guanine nucleotide exchange factor that activates ARFs through a mechanism that interferes with BFA action.
612116 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612116 Rev.1
Citations & References
View product citations for antibody "612116" on CiteAb
Development References (2)
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Claude A, Zhao BP, Kuziemsky CE, et al. GBF1: A novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5. J Cell Biol. 1998; 146(1):71-84. (Biology). View Reference
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Mansour SJ, Herbrick JA, Scherer SW, Melancon P. Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24. Genomics. 1998; 54(2):323-327. (Biology). View Reference
612116 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.