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Purified Mouse Anti-CDC42
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human,Mouse,Dog (Tested in Development)
Mouse IgG1
Human CDC42 aa. 1-191
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
22 kDa
250 µg/ml
AB_398244
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610928 Rev. 1
Antibody Details
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44/CDC42

Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae. CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis. CDC42 and Rac1, a Ras-related GTPase, activate MEKK1, a JNK kinase kinase, which leads to the activation of several downstream components of the MAP kinase cascade leading to activation of PAK65, a CDC42- and Rac-binding protein. PAK65 interacts with CDC42/Rac1, mediates their interaction with MEKK1, and enhances MEKK1 catalytic activity. Chronic activation of CDC42 has been shown to induce malignant cellular transformation. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody. BD Pharmingen has not performed such cross-reactivity testing.

610928 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610928 Rev.1
Citations & References
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View product citations for antibody "610928" on CiteAb

Development References (5)

  1. Brandt D, Gimona M, Hillmann M, Haller H, Mischak H. Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway. J Biol Chem. 2002; 277(23):20903-20910. (Clone-specific: Western blot). View Reference
  2. Habas R, Dawid IB, He X. Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation. Genes Dev. 2003; 17(2):295-309. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  3. Munemitsu S, Innis MA, Clark R, McCormick F, Ullrich A, Polakis P. Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42. Mol Cell Biol. 1990; 10(11):5977-5982. (Biology). View Reference
  4. Shinjo K, Koland JG, Hart MJ, et al. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. Proc Natl Acad Sci U S A. 1990; 87(24):9853-9857. (Biology). View Reference
  5. Zugasti O, Rul W, Roux P, et al. Raf-MEK-Erk cascade in anoikis is controlled by Rac1 and Cdc42 via Akt. Mol Cell Biol. 2001; 21(19):6706-6717. (Clone-specific: Western blot). View Reference
View All (5) View Less
610928 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.