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      Purified Mouse Anti-Human RIP with Control

      BD Pharmingen™ Purified Mouse Anti-Human RIP with Control

      (RUO)
      Purified Mouse Anti-Human RIP with Control
      Western blot analysis of RIP. Lysate from Jurkat cells was probed with anti-RIP (clone G322-2, Comp. No. 51-6559GR) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). RIP is identified as a band of 74 kDa.
      Purified Mouse Anti-Human RIP with Control Purified Mouse Anti-Human RIP with Control
      Western blot analysis of RIP. Lysate from Jurkat cells was probed with anti-RIP (clone G322-2, Comp. No. 51-6559GR) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). RIP is identified as a band of 74 kDa.
      Product Details
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      BD Pharmingen™
      Human (QC Testing)
      Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
      RUO
      AB_394015


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Recommended Assay Procedures

      Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.  Additional control lysate (Cat. No. 611451) is sold separately.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      551042 Rev. 2
      Components
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      Description Quantity/Size Part Number EntrezGene ID
      Purified Mouse Anti-Human RIP 50 µg (3 ea) 51-6559GR N/A
      Jurkat Cell Lysate 50 µg (1 ea) 51-16526N N/A
      551042 Rev. 2
      Citations & References
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      Development References (1)

      1. Stanger BZ, Leder P, Lee TH, Kim E, Seed B. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell. 1995; 81(4):513-523. (Biology). View Reference
      551042 Rev. 2

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.