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Purified Mouse Anti-Rb2
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human Rb2 aa. 26-367
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
130 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to for technical protocols.
610262 Rev. 1
Antibody Details
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The pRb2 protein shows a high degree of identity with pRb and the related p107 protein. Both pRb and p107 bind to the adenovirus E1A, SV40 large T antigen, and papillomavirus E7 viral proteins. This binding initiates the release of transcription factors which are required for the expression of cell cycle-regulated genes. pRb, pRb2, and p107 interact with a conserved motif in these three viral proteins. In the E1A protein, this area is known as transforming domain 2, which is required for growth activation. The E1A-binding domain in pRb and p107 is a conserved motif known as the "pocket region" which consists of conserved A and B regions separated by non-conserved spacers of different sizes in pRb and p107. It is the pocket regions of pRb and p107 that associate with the E2F transcription factor. pRb2 also contains the conserved pocket region suggesting that it has functional similarities to pRb and p107.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610262 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610262 Rev.1
Citations & References
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Development References (5)

  1. Fusaro G, Wang S, Chellappan S. Differential regulation of Rb family proteins and prohibitin during camptothecin-induced apoptosis. Oncogene. 2002; 21(29):4539-4548. (Clone-specific: Gel shift, Immunoprecipitation, Western blot). View Reference
  2. Laplantine E, Rossi F, Sahni M, Basilico C, Cobrinik D. FGF signaling targets the pRb-related p107 and p130 proteins to induce chondrocyte growth arrest. J Cell Biol. 2002; 158(4):741-750. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  3. Mayol X, Grana X, Baldi A, Sang N, Hu Q, Giordano A. Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain. Oncogene. 1993; 8(9):2561-2566. (Biology). View Reference
  4. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Clone-specific: Immunofluorescence). View Reference
  5. Yeung RS, Bell DW, Testa JR, et al. The retinoblastoma-related gene, RB2, maps to human chromosome 16q12 and rat chromosome 19. Oncogene. 1993; 8(12):3465-3468. (Biology). View Reference
View All (5) View Less
610262 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.