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BD Transduction Laboratories™ Purified Mouse Anti-P-Cadherin
Clone 56/P-Cadherin (RUO)
Western blot analysis of P-Cadherin on A431 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-P-Cadherin antibody.
Immunofluorescent staining of A431 cells.
Western blot analysis of P-Cadherin on A431 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-P-Cadherin antibody.
Immunofluorescent staining of A431 cells.
Product Details
BD Transduction Laboratories™
Human (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human P-Cadherin aa. 72-259
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
120 kDa
250 µg/ml
AB_2077667
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610228 Rev. 1
Antibody Details
56/P-Cadherin
Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Cadherins are similar in their domain structure, Ca2+ and protease sensitivity, and molecular weight. However, these proteins have distinct tissue expression patterns and immunological reactivities. P-Cadherin is localized in placenta while E-Cadherin and N-Cadherin are found in epithelial and neural tissues, respectively. P-Cadherin is an 829 amino acid polypeptide with a putative signal peptide and precursor region, an extracellular domain containing several internal repeats, and a highly hydrophobic transmembrane region. The cytoplasmic domain provides a link to the cytoskeleton through the associated catenin proteins.
610228 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610228 Rev.1
Citations & References
View product citations for antibody "610228" on CiteAb
Development References (5)
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Bailey T, Biddlestone L, Shepherd N, Barr H, Warner P, Jankowski J. Altered cadherin and catenin complexes in the Barrett's esophagus-dysplasia-adenocarcinoma sequence: correlation with disease progression and dedifferentiation. Am J Pathol. 1998; 152(1):135-144. (Clone-specific: Immunohistochemistry). View Reference
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Holsinger LJ, Ward K, Duffield B, Zachwieja J, Jallal B. The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn). Oncogene. 2002; 21(46):7067-7076. (Clone-specific: Western blot). View Reference
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Kantak SS, Kramer RH. E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells. J Biol Chem. 1998; 273(27):16953-16961. (Clone-specific: Western blot). View Reference
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Nose A, Nagafuchi A, Takeichi M. Isolation of placental cadherin cDNA: identification of a novel gene family of cell-cell adhesion molecules. EMBO J. 1987; 6(12):3655-3661. (Biology). View Reference
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Tao YS, Edwards RA, Tubb B, Wang S, Bryan J, McCrea PD. beta-Catenin associates with the actin-bundling protein fascin in a noncadherin complex. J Cell Biol. 1996; 134(5):1271-1281. (Clone-specific: Immunofluorescence). View Reference
610228 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.